cytostab {CytoGLMM} | R Documentation |
Evaluate parameter stability with respect to gating sheme
cytostab( df_samples_subset, protein_names, condition, group = "donor", cell_n_min = Inf, cell_n_subsample = 0 )
df_samples_subset |
Data frame or tibble with proteins counts, cell condition, and group information |
protein_names |
A vector of column names of protein to use in the analysis |
condition |
The column name of the condition variable |
group |
The column name of the group variable |
cell_n_min |
Remove samples that are below this cell counts threshold |
cell_n_subsample |
Subsample samples to have this maximum cell count |
A data frame
set.seed(23) df <- generate_data() protein_names <- names(df)[3:12] df <- dplyr::mutate_at(df, protein_names, function(x) asinh(x/5)) stab <- CytoGLMM::cytostab(df, protein_names = protein_names, condition = "condition", group = "donor") stab